Hidrológiai Közlöny 2009 (89. évfolyam)
6. szám - L. Hidrológiai Napok: "A hazai hidrobiológia ötven éve" Tihany, 2008. október 1-3.
Molecular biological investigations on the bacterial communities of the water of the Harkány Spa thermal-wells Abstract: Bacterial communities from the sulfide containing thermal-waters of Harkány Spa were investigated by cultivation independcnt molecular biological methods. Samples from lukewarm and thermal wells were collected seasonally in 2006 and 2007. Following the filtering of the water samples, DNA was extracted from the biomass. 16S rDNA was amplified with PCR using universal eubacterial primers. Denaturing Gradient Gel Electrophoresis (DGGE) was applied to compare the community structures and the seasonal changes; clone library analysis was used to reveal the phylogenetic diversity. The DGGE profiles of the tepid wells showed the highest similarity and variability mainly among the spring samples of different origin was found. From four clone libraries 16 different species or genera were identified by sequence analysis. The majority of the clones of tepid wells belonged to E-Proteobacteria, Desulfocapsa sp. and Thiothrix spp., while dominant clones of the thermal well were affiliated with the thermophilic Sulfurihydrogenibium and Thiomonas. The identified taxa were related to obligáté and facultative chemolithotrophic bacteria, accordingly the investigated karstic waters can be characterized with seasonally variable bacterial populations participating in the sulfur-cycle. Keywords: Thermal-water, chemolithotrophic, sulfuric bacteria, DGGE, clone library. (Klossy Irén alkotása) 155 Irodalom Chen, X.-G., Geng, A.-L., Liang, D.T.: Isolation and characterization of sulphur-oxidizing Thiomonas sp. and its potential applications in biological deodorization. Lett. Appl. Microbiol. 39: 495-503, 2004 Engel, A.S., Porter, M.L., Stern, L.A., Quinlan, S.. Bennett, P C.: Bacterial diversity and ecosystem function of filamentous microbial mats from aphotic (cave) sulfidic springs dominated by chemolithoautotrophic „Epsilonproteobacteria". FEMS Microbiol. Ecol. 51.31-53, 2004. Finster, K„ Liesack, W., Thamdrup. B.: Elemental sulfur and thiosulfate disproportionation by Desulfocapsa sulfoxigens sp. nov., a new anaerobic bacterium isolated from maríné surface sediment. Appl. Environ. Microbiol. 64: 119-125, 1998. Inagaki, F., Takai, K., Nealson, H.K., Horikoshi, K.\ Sulfurovum lithotrophicum gen.nov., sp. nov., a növel sulfur-oxidizing chemolithoautotroph within the e-Proteobacteria isolated from Okinawa Trough hydrothermal sediments Int. J. Syst. Evol. Microbiol., 54, 1477-1482, 2004. Kodama, Y., Watanabe, K.: Sulfuricurvum kujiense gen. nov. sp. nov., a facultatively anaerobic, chemolithoautotrophic, sulfur-oxidizing bacterium isolated from an underground crude-oil storage cavity. Int. J. Syst. Evol. Microbiol. 54: 2297-2300, 2004. Moissl, C., Rudolph, C„ Huber, R. \ Natural communities of növel Archaea and Bacteria with a string-of-pearls-like moiphology: Molecular analysis of the bacterial partners. Appl. Environ. Microbiol. 68: 933-937,2002. Nakagawa, T„ Fukui, M.: Molecular characterization of community structures and sulfur metabolism within microbial streamers in japanese hot springs. Appl. Environ. Microbiol. 69: 7044-7057, 2003. Spring, S., Kampfer, P., Schleifer, K.H.: Limnobacter thiooxydans gen.nov., sp.nov., a növel thiosulfate-oxidizing bacterium isolated from freshwater lake sediment. Int. J. Syst. Evol. Microbiol. 51: 1463-1470, 2001 Suzuki.D.; Ueki.A.; Amashit.A.; Ueki.K.: Desulfopila aestuarii gen. nov., sp. nov., a Gram-negative, rod-like, sulfate-reducing bacterium isolated from an estuarine sediment in Japan. Int. J. Syst. Evol. Microbiol. 57. 520-526, 2007. Tsukasa, /., Kenichi, S.. Isao, Y„ Yoshinobu, N., Satoshi, O.: Thiovirga sulfuroxydans gen. nov., sp. nov., a chemolithoautotrophic sulfur-oxidizing bacterium isolated from a microaerobic waste-water biofilm. Int. J. Syst. Evol. Microbiol. 55: 1059-1064, 2005.