"Lippay János - Ormos Imre - Vas Károly" Tudományos Ülésszak előadásai 2007, Élelmiszertudomány

Élelmiszerbiztonság, mikrobiológia és biotechnológia szekció

Lippay János - Ormos Imre - Vas Károly Scientific Symposium 7-8. November 2007 Food Safety, Microbiology and Biotechnology TRANSPOSON ELEMENTS AND MYCELIAL COMPAT1BILITY GROUPS OF BOTRYTIS CINEREA IN THE EGER AND TOKAJ WINE REGIONS Kálmán Zoltán Váczy (vaczy@szbki-eger.hu)1 - Levente Karafftf - György J. Kövics3 - Lajos Gál1 -Noémi Rudó3 - Erzsébet Sándor' 1 Research Institute fór Viticulture and Enology; Eger 2 University of Debrecen, Department of Applied Microbiology and Genelics; Debrecen 3 University of Debrecen, Department of Plánt Protection: Debrecen The ascomycete Botryotiniafuckeliana (anamorph: Botrytis cinerea) is the causal agent of both the destructive grey mould (pourriture grise) as well as the noble rőt (pourriture noble), an infection that results in wines with a special quality and most pleasant bouquet. The development of grey mould on the grape berries is among the most striking viticultural phenomena with complex preconditions and implications. In Hungary, the Tokaj wine district has a reputation fór the production of these great sweet pourriture noble wines, called „aszú”. On the other hand, in the nearby Eger wine district it is the pourriture grise that usually causes serious losses. B. cinerea has been shown to possess two types of transposons: eláss I (called Boty) and eláss II (called Flipper). Studies on French and Chilean isolates revealed three types of isolates: (1) having both transposable elements (2) having no transposable elements, and (3) containing transposable element Boty alone. Isolates containing only the Flipper transposon element showed the highest ratio both in the Eger an in the Tokaj wine district. This genotype is extremely rare or absent in Francé and Chile. Characterization of groups of vegetatively (somatically) compatible individuals provides a powerful approach to subdividing a species intő diserete populations in fílamentous ascomycetous fungi. Mycelial incompatibility test was used to define mycelial compatibility groups (MCGs) of B. cinerea. Classifying all strains intő distinct groups was difficult, and both dark interaction line and mycelial-ffee space were observed in interactions. The existance of multiply MCGs indicates that hyphal fúsions are nőt common in the B. cinerea populations cinerea in the Eger and Tokaj wine regions. 99

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