Neurobiology 7. (1999)
1. szám - Preliminary notes
Kicsi et al. that the intensity of none of the bands decreased in the latter as compared to the former, argues against internalization of surface p-opioid receptors due to chronic morphine exposure. We conclude that DAMCK is able to bind irreversibly to p opioid receptors both in vivo and in vitro. Thus, it will be used to monitor the intracellular trafficking of the receptor in future experiments. Supported by the US-Hungarian joint fund TET-564 and OTKA T-016084 research grant. REFERENCES 1. M. Szűcs, S. Benyhe, A. Borsodi, G. Jancsó, J. Szécsi, M. Medzihradszky. Binding characteristics and analgesic activity of D-Ala^LeiE-enkephalin Chloromethyl Ketone. Life Sei. 32: 2777-2784 (1983). 2. M. Szűcs, K. Di Gleria, К. Medzihradszky. Melphalan Potently Substitute the NTerminal Tyr of D-AIa2-Leu5-enkephalin Methyl Ester. FEBS Letters 179: 87-90 (1985). 3. M. Szűcs, M. Belcheva, J. Simon, S. Benyhe, G. Tóth, J. Hepp, M. Wollemann, К. Medzihradszky. Covalent Labelling of Opioid Receptors with (3H)D-Ala2-Leu5- enkephalin Chloromethyl Ketone, 1. Binding Characteristics in Rat Brain Membranes. Life Sei. 41: 177-184(1987). 4. E. Varga, J. Hepp, S. Benyhe, J. Simon, K. Medzihradszky, A. Borsodi. Synthesis of 3HTYR-D-ALA-GLY-N(ME)PHE chloromethyl ketone - an opioid affinity label. Neuropeptides 12: 135-139 (1988). 5. M. Szűcs, C.J. Coscia. Differential coupling of opioid binding sites to guanine triphosphate binding regulatory proteins in subcelluiar fractions of rat brain. J. Neurosci. Res. 31: 565-572 ( 1992).