Acta Biologica 38. (1987)
2. szám - J. Szabad–M. Erdélyi–J. Szidonya: Characterization of Fs(2)1, a germ-line dependent dominant female sterile mutation of Drosophila
Acta Biologies Hungarica 38(2), pp. 257—266 (1987) CHARACTERIZATION OF FS(2)1, A GERM-LINE DEPENDENT DOMINANT FEMALE STERILE MUTATION OF DROSOPHILA J. Szabad, M. Erdélyi and J. Szidonya Institute of Genetics, Biological Research Center of the Hungarian Academy of Sciences, H-6701 Szeged, Hungary (Received 1987—03—25) Fs(2)l is a germ-line dependent dominant female sterile mutation of Drosophila melanogaster. Fs(2)l heterozygous females deposit very few abnormal eggs (collapsed, with malformed chorion). The degeneration of egg primorida starts around the end of egg maturation. Mitotic recombination mapping locates Fs(2)l in a distal region of the left arm of the 2nd chromosome. Fs(2)l is a good tool for studying germ-line functions (by the dominant female sterile technique) because the frequency of germ-line mosaicism exceeds 20% upon irradiation of adult females. Salivary gland polytene chromosomes of Fs(2)l and the revertant heterozygous larvae appear normal. Keywords: Dominant-female-sterile technique — germ-line mosaics — Drosophila INTRODUCTION A possible approach to the understanding of the genetic and developmental regulation of oogenesis, maternal effects and embryogenesis is to analyze the effects of mutations on germ-line cells. Mosaic egg primordia are usually created, in which mutant germ-line cells (the oocyte and 15 nurse cells) are surrounded by phenotypically wild-type follicular cells. When the development of the mosaic egg primordia or the resultant embryo is affected, the mutation is considered to be germ-line dependent and it can be deduced that the gene identified by the mutation has germ-line functions. Send offprint requests to: Dr. J. Szabad, Institute of Genetics, Biological Research Center, H—6701 Szeged, P.O.Box 521, Hungary